Experimental Conditions

Definition

Experimental conditions are types of information you can add to your run and use in normalizations, automatic analysis, and visualizations. For each well of each plate, you can add a value linked to an experimental condition.

For example, to define the lineage, create the experimental condition Lineage and assign a lineage value to each well.

In this documentation, Experimental Condition refers to the information type (e.g., Lineage). Experimental Condition values refer to the value assigned to each well.

Accessing the Experimental Condition Feature

A button is available on almost every step of your run to access the Experimental Condition page, located in the header.

If you access Experimental Conditions before the Plate Mapping step, you can only view existing conditions, not add values. After this step, you can add, modify, or delete values.

Add Experimental Condition Values

To add a value:

• Select one or multiple plates in the Plate selection region using checkboxes.
• The header checkbox selects/unselects all plates in your run.
• The View button for each plate lets you see values for one plate and keep your selection.
• Use the filter to display only incomplete plates for this condition.
• Choose a filling mode:
  • Manual filling mode: Enter a value, select wells, and apply.
  • Layout filling mode: Select a layout to display and apply.
• To delete values, select wells and use the Clear button. You cannot delete values when a layout is displayed or if you used the View button.

While adding values, you can check other experimental conditions and plates. When the spinner disappears, you can continue editing values.

Retrieve Information from Sample

With the Retrieve from Sample button on the Experimental Condition page, you can choose the data to retrieve from Sample. For each type of information (Sample, Dose, Solvent, etc.), you can choose to import it or not. Currently, the following information can be retrieved from Sample:

• Batch name & alias
• Parent batch name & alias
• Batch name & Parent batch name
• Concentration
• Solvent
• Molecular weight
• Annotations

If information you want to retrieve is missing:

• Your plates may not be registered in Sample. Verify the barcode is registered in both Sample and Assay.
• Your plates may not have the information registered in Sample. Check your plate in Sample.

For Solvent and Annotations linked to your plates in Sample, you can register them in your custom Experimental Conditions.