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Tutorial - Serial Dilution with a Dose Layout

Introduction

In this tutorial, you will perform a dilution by row from a 96-well plate with a single column of filled wells (source concentration = 10 mM, Dilution Factor = 2) using a Dose Layout.

Prerequisites

Required permissions and setup

Sample Processing role is required to access this workflow.

Required system configuration:

  • Container Types: Greiner PP96V
  • Container Usage: Inventory
  • Solvent: DMSO
  • Worklist Generator and Parser: Standard (Well) (;)
  • The dilution layout named Dilution Factor 2 - 96 must exist in the system.

Plate Serial Dilution Layout

These should exist by default in the Sample application.

Items

Containers and locations created from Discngine Sample’s automation will be used:

  • A plate with a single column of filled wells (easily created with workflow “Plate creation from vials”) corresponding to column n°1. Automation is running during installation if “example_dataset” is set to true in the configuration file.

Workflow

Job Initialization

Create a new job and select the Plate Serial Dilution workflow.

Requesting

  • Select Greiner PP96V as the Target Plate Type
  • Specify Request from Text
  • Enter the name of your plate (Prerequisite: a plate with empty wells except in its first column). In this example: P000000001.
  • Click the Add IDs button to confirm. The plate is listed in the added requests.
  • Click the Next button.

Task Requesting

  • Dilution Template = Dose Layout
  • Dose Layout = Dilution Factor 2 - 96
  • Dilution by = Row
  • Initial Column Index = 1
  • Solvent = DMSO
  • Click Add Task. One task is added (for all dilutions).
  • Click Next

Sourcing

The Sourcing page is used to check that the requested operations are compatible with the source plate (information in the Sourced column) and available.

  • Click Next

Processing

For processing, requested tasks are translated into a worklist that you can feed to your robotic equipment.

  • Select the Standard (Well) (;) equipment and set File Order to By Request
  • Click Generate worklist

A worklist file appears in the Worklist Generator area. It contains the requested tasks to execute. The Worklist Parser area expects a file containing the information about the tasks that were actually completed. By default, the generated file is set in the Worklist Parser. For this tutorial, we assume that every task was completed successfully, so we’ll keep this default file.

  • Click Copy Worklist
  • Click Next to propagate those changes in the system. The tasks are processed in the Sample application.

Storing

The Storing step allows you to assign a location to the items involved in the job. In this workflow, as the serial dilution is done within the plate, the input plate is the output plate.

By clicking on the plate barcode, you can access your plate details to check if the dilutions were performed.

  • Click Next to continue. Here, we won’t change the input plate's location. You can increment the Thawing Cycle count of the input plates if necessary.

Pipe & Close

The Pipe & Close step provides a summary page where you can review the job and optionally pipe it to another workflow. You can also pipe this job to another one, allowing you to immediately start a new job using the output or the input plates from this one. For this tutorial, click Close & Quit to finish the job.