Tutorial - In-Plate Dilution

Introduction¶

This tutorial will guide you step by step through the in-plate dilution workflow, from job initialization to final storage and closure.

Prerequisites¶

Required permissions and setup

Sample Reception role is required to access this workflow.

Required system configuration:

Plate Type Greiner PP96V,
Solvent Buffer/Medium From Registration

These should exist by default in the Sample application.

Workflow¶

Job Initialization¶

Create a new job and select the In-Plate Dilution workflow.

Requesting¶

Select Plate as Source Type
Select Greiner PP96V as Plate Type
Specify Request from Text
Copy/paste the plate names in the text area: PTEST001:PTEST002 (Plate names can be separated by colons or line breaks.)
Click Add IDs to confirm. The plates are listed in the added requests.
Click Next.

Task Requesting¶

Added volume is 5 µL
Select Buffer/Medium From Registration as Solvent
Click Next

Storing¶

The Storing step allows you to assign a location to the items involved in the job. First, for the input plates (plates already existing used in the job).

Here, we won’t change the input vials location.

Click Next

You can increment the Thawing Cycle count of the input plates if necessary.

Pipe & Close¶

The Pipe & Close step provides a summary page where you can review the job and optionally pipe it to another workflow. You can also pipe this job to another one, allowing you to immediately start a new job using the input plates from this one.

For this tutorial, click Close & Quit to finish the job.