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Tutorial - Vial Solid Splitting

Introduction

In this tutorial, you will get an overview of the Vial Solid Splitting workflow to create both solid and liquid vials by running two different jobs covering different aspects of the workflow.

This workflow is usually performed manually, so no robotic worklist will be produced.

Example of vial solid splitting workflow

Prerequisites

Required permissions and setup

Sample Processing role is required to access this workflow.

Required system configuration:

  • Vial Type: 1 Dram Vial
  • Container Usage: Inventory
  • Solvent: DMSO
  • Worklist Generator and Parser: Standard (Container) (;)

These should exist by default in the Sample application.

Items

Containers and locations created from Discngine Sample’s automation will be used:

  • Five vials in solid state (100 mg, batch with molecular weight)
  • One vial in solid state (10 mg, batch without molecular weight)
  • One vial in liquid state (100 μL, 10 mM)
  • Location: Paris Automation runs during installation if example_dataset is set to true in the configuration file.

Workflow

Job Initialization

Create a new job and select the Vial Solid Splitting workflow..

Requesting

  • Select Vial as Source Type
  • Specify Request from Text
  • Copy and paste the vial names in the text area: VTEST001:VTEST002:VTEST003:VTEST004:VTEST011 Vial names can be separated by colons or line breaks.

Info

Test vials 1 to 10 are solid, 11 to 20 are liquid. Two of the requested vials are in a liquid state and cannot be processed by this workflow.

  • Click the Add IDs button to confirm. The vials are listed in the added requests.
  • Click the Next button. A warning message indicates that the incompatible vial will be ignored during processing. Click OK to continue.

Task Requesting

You will create two solid vials and two liquid vials.

  • Choose From Selection for the Source Vial option and select the first two vials (VTEST001 and VEST002). Define a task to create solid vials from them:
    • Target Type: 1 Dram Vial
    • Target Usage: Inventory
    • Number of Copy: 1
    • Target State: Dry
    • Target Amount: 1 mg

  • Click Add this task. One task is added.

  • Now, select the two other vials (VTEST003 and VEST004) and make a liquid copy using a mass concentration:

    • Target Type: 1 Dram Vial
    • Target Usage: Inventory
    • Number of Copy: 1
    • Target State: Solution
    • Target Volume: 1 mL
    • Target Concentration: 1 mg/mL
    • Solvent: DMSO Click Add this task. We now have 2 tasks defined.

  • Click the Next button.

Sourcing

The Sourcing page will confirm that the requested operations are compatible with the source vials (see the Sourced column).

Info

sSolubilizing a solid using a molar concentration is not possible if we don’t have its molecular weight.

  • Click the Next button.

Processing

This workflow usually involves manual operation to weigh the solid batch to transfer. The amount of batch weighed can be slightly different from the theoretical task. On this page, fill in for each task the quantity that was actually picked from the source.

Scan the source container and the destination container (or enter their barcode after clicking the Add Quantities button). Fill in the requested quantity and press Enter again to validate.

Info

This step can be automated if a laboratory balance is connected to the system.

  • Click the Next button when all rows are processed.

Storing

The Storing step allows you to assign a location to the items involved in the job.

  • Click the Assign Location to Selected button.
  • Select Paris and click the Select button to confirm your choice.
  • Click the Next button to continue to the input vials. (Here, we won’t change the input vials' location.)
  • Click the Next button. (You can increment the Thawing Cycle count of the input vials if necessary.)

Pipe & Close

The Pipe & Close step provides a summary page where you can review the job and optionally pipe it to another workflow. You can also pipe this job to another one, allowing you to immediately start a new job using the output or the input vials from this one.

For this tutorial, click the Close & Quit button to finish the job.