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Tutorial - Vial Solubilization

Introduction

In this tutorial, you will add solvent to a solid batch in a vial to convert it to liquid state.

Prerequisites

Required permissions and setup

Sample Processing role is required to access this workflow.

Required system configuration:

  • Vial Type 1 Dram Vial,
  • Container Usage Inventory,
  • Solvent DMSO,
  • Worklist Generator and Parser: Standard (Container) (;)

These should exist by default in the Sample application.

Items

Containers and locations created from Discngine Sample’s automation will be used:

  • 1 vial in solid state (10 mg, batch with molecular weight)
  • 1 vial in solid state (10 mg, batch without molecular weight)
  • 1 vial in liquid state (100 μL, 10 mM)
  • Location: Paris

Automation runs during installation if "example_dataset" is set to true in the configuration file.

Workflow

Job Initialization

Create a new job and select the Vial Solubilization workflow.

Requesting

  • Select Vial as Source Type
  • Specify Request from Text
  • Copy/paste the vial names in the text area: VTEST001:VTEST011:VTEST_NMW_001

Vials names can be separated by colons or line breaks.

Info

Test vials 1 to 10 are solid, 11 to 20 are liquid. For test vials, the NMW tag stands for No Molecular Weight. This vial contains a solid batch with no molweight defined. One of the requested vial is in a liquid state and cannot be processed by this workflow. The vial with no molecular weight defined can be processed in the workflow but a warning message indicates that we won’t be able to request a dilution based on a molar concentration for it.

  • Click Add IDs to confirm. The Vials are listed in the added requests.
  • Click Next. A warning message indicates that the invalid vial will be ignored during processing, click OK to continue.

Task Requesting

  • Choose Source Vial: All Vials
  • Using Target: Concentration
  • Target Concentration: 1 M
  • Solvent: DMSO
  • Click Add Task.

An error message appears. As stated in the previous step, solubilization using a molar concentration isn’t compatible with the vial VTEST_NMW_001.

  • Change the Source Vial option from All Vials to From Selection
  • Pick the vial VTEST_NMW_001 in the shuttle selection
  • Define a task that doesn’t use molar concentration:
    • Using Target: Volume
    • Target Volume: 100 μL
    • Solvent: DMSO
  • Click Add Task.
  • Click Next.

Sourcing

The Sourcing page will confirm that the requested operations are compatible with the source vials (see the Sourced column).

  • Click Next.

Processing

For processing, requested tasks are translated into a worklist that you can feed to your robotic equipment.

  • Select the Standard (Container) (;) equipment and set File Order to By Request
  • Click Generate worklist.

A worklist file appears in the Worklist Generator area. It contains the requested tasks to execute. The Worklist Parser area expects a file containing information about the tasks that were actually completed. By default, the generated file is set in the Worklist Parser. For this tutorial, we assume that every task was completed successfully, so we’ll keep this default file.

  • Click Copy Worklist.
  • Click Next to propagate these changes in the system.

The tasks are processed in the Sample application.

Storing

For this workflow, there is no output container (transformations were directly applied to the source container). Do not change the location of the vials used.

  • Click Next.

You can increment the Thawing Cycle count of the input vials if necessary.

Pipe & Close

The Pipe & Close step provides a summary page where you can review the job and optionally pipe it to another workflow. You can also pipe this job to another one, allowing you to immediately start a new job using the output or the input vials from this one.

For this tutorial, click Close & Quit to finish the job.