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Tutorial - Combine Replicates

Introduction

In this tutorial, you will replicate the content of a single 96-well plate into a 384-well plate as illustrated below.

Plate Reformatting combine replicates

Prerequisites

Required permissions and setup

Sample Processing role is required to access this workflow.

Required system configuration:

  • Container Types: Greiner PP96V,Greiner PP384V,
  • Container Usage Inventory,

These should exist by default in the Sample application.

Items

Containers and locations created from Discngine Sample’s automation will be used:

  • 96-well plate PTEST001
  • Location Paris Automation is running during installation if “example_dataset” is set to true in the configuration file.

Workflow

Job Initialization

Create a new job and select the Plate Reformatting workflow.

Requesting

  • Select Plate as Source Type
  • Select Generate New Barcode as Target Plate Barcode
  • Choose Greiner PP384V as Target Plate Type
  • Specify Request from Text
  • Copy/paste the plate name in the text area: PTEST001
  • Click Add IDs to confirm. The plate is listed in the added requests.
  • Click Next

Task Requesting

  • Choose Reformatting Mode = Combine replicates
  • Target Replicate Count = 4
  • Target Usage = Inventory
  • Select Number of Copy = 1
  • Target Volume = 30 μl
  • Dilution = Target
  • Dilution Mode = Dilution Factor
  • Dilution Factor = 10
  • Buffer (Solvent) = DMSO
  • Offset Volume = 0%
  • Offset Solvent = 0%

The “Combine Replicate” option will create replicates for each sample of the source plate(s). Here, the process will start from the first well of PTEST001 and create 4 replicates in the corresponding quadrants of the target 384-well plate (see figure in Introduction). Compared to the other option (see the Reformatting – Combine Source tutorial), it allows you to replicate samples during a reformatting.

  • Click Add Task
  • Click Next

Sourcing

Plates are successfully sourced if the requested tasks are compatible (e.g. sufficient volume). The plate “PTEST001” should be sourced.

  • Click Next

Processing

  • Select the Standard (Container) (;) equipment and By Request for File Order
  • Click Generate worklist

A worklist file appears in the Worklist Generator area. It contains the requested tasks to execute. The Worklist Parser area expects a file containing the information about the tasks that were actually completed. By default, the generated file is set in the Worklist Parser. For this tutorial, we assume that every task was completed successfully, so we’ll keep this default file.

  • Click Copy Worklist
  • Click Next to propagate those changes in the system. The tasks are processed in the Sample application.

Storing

The Storing step allows you to assign a location to the items involved in the job. First, for the output plate (plate created during the job).

  • Click Assign Location to Selected
  • Select Paris > HTS Lab and click Select to confirm your choice.
  • Click Next to continue to the input plates. Here, we won’t change the input plates location.
  • Click Next You can increment the Thawing Cycle count of the input plates if necessary.

Pipe & Close

The Pipe & Close step provides a summary page where you can review the job and optionally pipe it to another workflow. You can also pipe this job to another one, allowing you to immediately start a new job using the output or the input plates from this one.

For this tutorial, click the Close & Quit button to finish the job.